Evolutionary Analysis of Pre-S/S Mutations in HBeAg-Negative Chronic Hepatitis B With HBsAg < 100 IU/ml

Background: Hepatitis B surface antigen (HBsAg) and viral load are important clinical indicators for antiviral therapy. Few studies have evaluated sequence biomarkers predicting the risk of hepatocellular carcinoma (HCC) in stage, which show a low serological response (HBsAg &amp;lt; 100 IU/ml) high levels (HBV DNA &amp;gt; 2,000 IU/ml). This study aims to determine trend biological prevalence within pre-S/S regions special model inactive CHB infection. Methods: We used Sanger sequencing, quantitative HBV serology (HBeAg HBsAg), liver function index identify whether genome sequences associated with long-term further HCC progression Results: sequencing analysis 28 patients infectious pattern showed higher genetic diversity among four opening reading frames (ORFs) ( p 0.001). However, dN/dS ratios HBsAg pre-C/C experimental group no significantly different from those = 0.06), while lower polymerase HBxAg In addition, seven positively selected sites were identified pre-S1, five pre-S2, S, (128H/135Q/135R/139L/141P) “α” determinant. Conclusions: These mutations region might be phenotype expression, P possibly impacting loads. Increased across is also HBsAg. The cumulative evolutionary changes shows that facilitate immune evasion should monitored individually. Due similarity characteristics HCC, responses viremia may disease progression.